Agarose gel electrophoresis

NuPAGE Tris-acetate/acetate

NuPAGE Tris-glycine/MOPS

Bacterial cultures

Bacterial cultures, cryopreservation

Odyssey infra-red detection

Bacterial growth media

Oligofectamine

Bacterial transformation

BCA

p70S6K kinase assay

Blocking, immunofluorescence

Paraformaldehyde, immunofluorescence

Blocking, western

Passage of mammalian cells

Bradford

PCR amplification

Phospho-H3 stainnig for mitotic cells

Cell cycle analysis

Plasmid maxi-prep (CsCl method)

Cell cycle profile analysis by FACS

Plasmid maxi-prep (Qiagen)

Cell fixation, and permeabilization, immunofluorescence

Plasmid mini-prep (Qiagen)

Cell synchronization agenents

PLK1 kinase assay

Centrosome amplification assay

Ponceau S staining

Centrosome isolation

Chemiluminescence

Rac1 and cdc42 GTPase assays

Coomasie staining

Retroviral production

Cytokinesis assay

Retroviral transduction

Rheb GTPase assay

Dephosphorylation of linearized DNA

Rho GTPase assay

Double-thymidine block

RhoA staining, immunofluorescence

Fluorophores

Silver staining, protein gels

Fugene 6

siRNA-mediated silencing

Site-directed mutagenesis (PCR)

G2/M synchronization

Growth media of common cell lines

Thawing of bacterial cultures

Thawing of mammalian cells

In-cell western

Transfection, Fugene6

Transfection, Lipofectamine 2000

Kinase assays

Transfer or proteins

TransIT-TKO

Lentiviral production

Trichloro-acetic acid (TCA) fixation, immunofluorescence

Lentiviral transduction

Tris-glycine-SDS, electrophoresis

Ligation

Tris-glycine-SDS PAGE, preparation of gels

Lipofectamine 2000

Mammalian cells, cryopreservation

Mammalian cell lysis

Methanol fixation, immunofluorescence

Microtubule binding assay

mTORC2 kinase assay