Agarose gel electrophoresis

NuPAGE Tris-acetate/acetate

NuPAGE Tris-Glycine/MOPS

Bacterial cultures

Bacterial cultures, Cryopreservation

Odyssey infrared detection

Bacterial growth media

Oligofectamine

Bacterial transformation

BCA

p70S6K kinase assay

Blocking, immunofluorescence

Paraformaldehyde, immunofluorescence

Blocking, western

Passage of mammalian cells

Bradford

PCR amplification

Phospho-H3 staining for mitotic cells

Cell cycle analysis

Plasmid maxi-prep (CsCl method)

Cell cycle profile analysis by FACS

Plasmid maxi-prep (Qiagen)

Cell fixation, and permeabilization, immunofluorescence

Plasmid mini-prep (Qiagen)

Cell synchronization agenents

PLK1 kinase assay

Centrosome amplification assay

Ponceau S staining

Centrosome isolation

Chemilluminescence

Rac1 and cdc42 GTPase assays

Coomasie staining

Retroviral production

Cytokinesis assay

Retroviral transduction

Rheb GTPase assay

Dephosphorylation of linearized DNA

Rho GTPase assay

Double-thymidine block

RhoA staining, immunofluorescence

Fluorophores

Silver staining, protein gels

Fugene 6

siRNA-mediated silencing

Site-directed mutagenesis (PCR)

G2/M synchronization

Growth media of common cell lines

Thawing of bacterial cultures

Thawing of mammalian cells

In-cell western

Transfection, Fugene 6

Transfection, Lipofectamine 2000

Kinase assays

Transfer or proteins

TransIT-TKO

Lentiviral production

Trichloro-acetic acid (TCA) fixation, immunofluorescence

Lentiviral transduction

Tris-Glycine-SDS, electrophoresis

Ligation

Tris-Glycine-SDS PAGE, preparation of gels

Lipofectamine 2000

Mammalian cells, Cryopreservation

Mammalian cell lysis

Methanol fixation, immunofluorescence

Microtubule binding assay

mTORC2 kinase assay